If genomic DNA has been inserted instead of the plasmid, what will happen?

A. It would lead to inactivation of the lacZ gene

B. The X-gal substrate would be broken down

C. The colonies formed are blue in color

D. The lacZ gene would be intact

Explanation: If genomic DNA is inserted instead of the plasmid the lacZ gene would be inactivated. The X-gal substrate won’t be broken down and thus the colonies formed would be white or off-white.

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