The greatest resolution in light microscopy can be obtained with ___________

A. Shortest wavelength of visible light used

B. Longest wavelength of visible light used

C. An objective with a minimum numerical aperture

D. Shortest wavelength of visible light used and an objective with the maximum numerical aperture

Explanation: The relationship between numerical aperture (NA) and resolution is:-

Resolution (d) = wavelength / 2(NA)

Thus maximum resolution is obtained with the shortest wavelength of visible light and an objective with the maximum NA.

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