Showing posts with the label DNA SequencingShow All

In Maxam-Gilbert method, chemical used for cytosine alteration is _______.

7/04/2022 03:58:00 pm

A. hydrazine B. piperidine C. dimethyl sulfate D. formic acid

The chemical used for strand cleavage in Maxam-Gilbert method is ___________.

7/04/2022 03:55:00 pm

A. piperidine B. hydrazine C. formic acid D. DMSO

ddNTP is different from dNTP in having ___________.

7/04/2022 03:44:00 pm

A. H in place of OH in 3 position of dNTP B. OH in place of H in 3 position of dNTP C.…

Sanger sequenced the first complete genome of __________.

7/03/2022 03:59:00 pm

A. T4 phage B. C. elegans C. TMV D. ΦX174

Automated DNA sequencing is an improvement of Sanger's method where ___________.

7/03/2022 03:57:00 pm

A. ddNTPs are used for chain termination B. Fluroscently labeled dNTPs are used for ch…

Frederick Sanger won the Nobel prize in 1980 for sequencing phage ΦX174. He was already awarded the Nobel prize in 1958 for _____________.

7/03/2022 03:53:00 pm

A. discovery of Sanger's method B. elucidating the nucleotide sequence of insulin …

In automated DNA sequencing ____________.

7/03/2022 03:47:00 pm

A. radiolabelled dNTPs are used B. radiolabelled ddNTPs are used C. fluorescently labe…

How many ddNTPs are used in Sanger's method?

7/03/2022 03:43:00 pm

A. 1 B. 2 C. 3 D. 4

Which of the following is called as dideoxynucleotide chain terminator method?

7/03/2022 03:42:00 pm

A. Sanger sequencing B. Maxam-Gilbert method C. Edman's method D. Automated DNA se…

Which of the following is the chemical sequencing method?

7/03/2022 03:39:00 pm

A. DNA chip sequencing B. Edman's method C. Sanger sequencing D. Maxam-Gilbert met…

The function of urea in the sequencing gels is to promote adherence.

7/02/2022 02:13:00 pm

A. True B. False Explanation: The sequencing gels contain 6-20% acrylamide and 7 M ure…

What is the molarity of urea used in sequencing gels?

7/02/2022 02:11:00 pm

A. 1 M B. 3 M C. 5 M D. 7 M

A sequencing gel is a ________________ gel.

7/02/2022 02:05:00 pm

A. Toxic B. Highly-polymerized C. High resolution D. Low resolution

Prior to getting electrophoresed in the sequencing gel, DNA is ____________.

7/02/2022 02:01:00 pm

A. Purified B. Denatured C. Synthesized D. Fragmented

How many types of deoxynucleoside triphosphates are used in Sanger sequencing?

7/02/2022 01:59:00 pm

A. 1 B. 2 C. 3 D. 4

Which of the following act as chain terminator?

7/02/2022 01:49:00 pm

A. Exogenous B. DNA C. Deoxynucleotides D. Dideoxynucleotides

What is the main enzyme component of Sanger sequencing?

7/02/2022 01:46:00 pm

A. Helicase B. Polymerase C. Nuclease D.  Gyrase

Sequence of which of the following cannot be determined using the Maxam Gilbert method?

7/02/2022 01:38:00 pm

A. Bacteria B. Plants C. Bacteriophage T7 D. Plasmid Explanation: Although Maxam Gilbe…

Which type of DNA cleavage is done in the Maxam Gilbert method?

7/02/2022 01:36:00 pm

A. Edge B. Interstitial C. Base-specific D. Gene-specific Explanation: The Maxam Gilb…

Plus and minus sequencing is the other name for Sanger sequencing.

7/02/2022 01:31:00 pm

A. True B. False Explanation: Plus and minus sequencing was used by Sanger in 1977 to …