Showing posts with the label Molecular biologyShow All

Which of the following RNA serves as adapter molecule during protein synthesis?

7/27/2022 03:59:00 pm

A. rRNA B. mRNA C. tRNA D. hnRNA

Amino acids are attached to the ?

7/27/2022 03:58:00 pm

A. acceptor arm of tRNA B. anti-codon arm of tRNA C. codon arm of tRNA D. none of thes…

RNA is the genetic material in ?

7/27/2022 03:54:00 pm

A.Viruses only B. In some viruses and some prokaryotic C. In some viruses and some pro…

In Maxam-Gilbert method, chemical used for cytosine alteration is _______.

7/04/2022 03:58:00 pm

A. hydrazine B. piperidine C. dimethyl sulfate D. formic acid

The chemical used for strand cleavage in Maxam-Gilbert method is ___________.

7/04/2022 03:55:00 pm

A. piperidine B. hydrazine C. formic acid D. DMSO

ddNTP is different from dNTP in having ___________.

7/04/2022 03:44:00 pm

A. H in place of OH in 3 position of dNTP B. OH in place of H in 3 position of dNTP C.…

Sanger sequenced the first complete genome of __________.

7/03/2022 03:59:00 pm

A. T4 phage B. C. elegans C. TMV D. ΦX174

Automated DNA sequencing is an improvement of Sanger's method where ___________.

7/03/2022 03:57:00 pm

A. ddNTPs are used for chain termination B. Fluroscently labeled dNTPs are used for ch…

Frederick Sanger won the Nobel prize in 1980 for sequencing phage ΦX174. He was already awarded the Nobel prize in 1958 for _____________.

7/03/2022 03:53:00 pm

A. discovery of Sanger's method B. elucidating the nucleotide sequence of insulin …

In automated DNA sequencing ____________.

7/03/2022 03:47:00 pm

A. radiolabelled dNTPs are used B. radiolabelled ddNTPs are used C. fluorescently labe…

How many ddNTPs are used in Sanger's method?

7/03/2022 03:43:00 pm

A. 1 B. 2 C. 3 D. 4

Which of the following is called as dideoxynucleotide chain terminator method?

7/03/2022 03:42:00 pm

A. Sanger sequencing B. Maxam-Gilbert method C. Edman's method D. Automated DNA se…

Which of the following is the chemical sequencing method?

7/03/2022 03:39:00 pm

A. DNA chip sequencing B. Edman's method C. Sanger sequencing D. Maxam-Gilbert met…

The function of urea in the sequencing gels is to promote adherence.

7/02/2022 02:13:00 pm

A. True B. False Explanation: The sequencing gels contain 6-20% acrylamide and 7 M ure…

What is the molarity of urea used in sequencing gels?

7/02/2022 02:11:00 pm

A. 1 M B. 3 M C. 5 M D. 7 M

A sequencing gel is a ________________ gel.

7/02/2022 02:05:00 pm

A. Toxic B. Highly-polymerized C. High resolution D. Low resolution

Prior to getting electrophoresed in the sequencing gel, DNA is ____________.

7/02/2022 02:01:00 pm

A. Purified B. Denatured C. Synthesized D. Fragmented

How many types of deoxynucleoside triphosphates are used in Sanger sequencing?

7/02/2022 01:59:00 pm

A. 1 B. 2 C. 3 D. 4

____________ is a chemically synthesized oligonucleotide.

7/02/2022 01:55:00 pm

A. Klenow fragment B. DNA C. Primer D. RNA

The Klenow fragment is basically a _______________.

7/02/2022 01:52:00 pm

A. DNA hybrid B. DNA polymerase C. RNA polymerase D. Promoter